An effective method to enhance transient gene expression by transfecting high-density CHO cells
Yu-shun Fu, Xue-lian Han, Wei-dong Li, Lin-lin Shan, Shao-lei Geng, Chun-liu Mi, Wei-hua Dong, Tian-yun Wang, Xiao-yin Wang · 2025
Read An effective method to enhance transient gene expression by transfecting high-density CHO cells by Yu-shun Fu, Xue-lian Han, Wei-dong Li, Lin-lin Shan, Shao-lei Geng, Chun-liu Mi, Wei-hua Dong, Tian-yun Wang, Xiao-yin Wang (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12748440.txt.
Description
Yu-shun Fu and co-authors report a recipe for making lab-grown proteins faster and in larger amounts. Their 2025 paper works with CHO cells, the standard host for making medical proteins. A quick method called transient gene expression is cheap and fast, but it often yields too little protein, so the team set out to raise the yield.
They tuned the carrier DNA step by step, adjusting parts that control how genes switch on, and then tuned the delivery itself. The best mix used a set DNA dose, a fixed ratio of DNA to the carrier chemical, and a repeat delivery round. Gene delivery reached about three-quarters of the cells while keeping most cells alive. Two test proteins rose more than fourfold, and two medical proteins, a collagen and a blood protein, rose by similar amounts.
Catalog record
| CREATOR | Yu-shun Fu, Xue-lian Han, Wei-dong Li, Lin-lin Shan, Shao-lei Geng, Chun-liu Mi, Wei-hua Dong, Tian-yun Wang, Xiao-yin Wang |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |