Testing antimicrobial peptides inhibiting protein synthesis in living E. coli and K. pneumoniae using bio-orthogonal non-canonical amino-acid tagging
Luigi de Pascale, Agnese D’Amore, Adriana Di Stasi, Martino Morici, Sabrina Pacor, Alessandro Tossi, Thuy Duong Pham, Attilio Fabbretti, Daniel N. Wilson, Mario Mardirossian, Marco Scocchi · 2025
Read Testing antimicrobial peptides inhibiting protein synthesis in living E. coli and K. pneumoniae using bio-orthogonal non-canonical amino-acid tagging by Luigi de Pascale, Agnese D’Amore, Adriana Di Stasi, Martino Morici, Sabrina Pacor, Alessandro Tossi, Thuy Duong Pham, Attilio Fabbretti, Daniel N. Wilson, Mario Mardirossian, Marco Scocchi (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12748266.txt.
Description
De Pascale and co-authors test a tagging method for studying germ-killing peptides in a 2025 paper. Some peptides halt protein building inside bacteria instead of tearing the cell wall. The tagging method, called BONCAT, marks fresh proteins in living cells. The team paired it with a dye test that shows whether the wall stays whole.
In two germ species, the peptides stopped protein building within ten minutes while walls stayed intact. The method cleanly split peptides that block building from those that break walls. Unlike a standard drug, the peptides kept working after they were washed away. The authors say the approach can sort how other germ killers act as well.
Catalog record
| CREATOR | Luigi de Pascale, Agnese D’Amore, Adriana Di Stasi, Martino Morici, Sabrina Pacor, Alessandro Tossi, Thuy Duong Pham, Attilio Fabbretti, Daniel N. Wilson, Mario Mardirossian, Marco Scocchi |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 55 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |