PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments
Mario Del Rosario, Estibaliz Gómez-de-Mariscal, Leonor Morgado, Raquel Portela, Guillaume Jacquemet, Pedro M Pereira, Ricardo Henriques · 2025
Read PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments by Mario Del Rosario, Estibaliz Gómez-de-Mariscal, Leonor Morgado, Raquel Portela, Guillaume Jacquemet, Pedro M Pereira, Ricardo Henriques (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12738901.txt.
Description
Mario Del Rosario and six co-authors present a toolkit for measuring light damage in live cell microscopy in a 2025 paper. Watching living cells under a microscope means bathing them in light, which can quietly stress or harm them and spoil results. Their framework pairs a standard exposure protocol with machine learning analysis of cell behavior, without any labels.
By tracking division timing, cell size, and activity, the toolkit detects stress that depends on light color and dose. Near ultraviolet light delayed cell division at surprisingly low doses, while longer wavelengths needed much more light for similar harm. The authors say the method lets labs set firm limits on acceptable damage and tune imaging to keep samples healthy.
Catalog record
| CREATOR | Mario Del Rosario, Estibaliz Gómez-de-Mariscal, Leonor Morgado, Raquel Portela, Guillaume Jacquemet, Pedro M Pereira, Ricardo Henriques |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 52 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |