Targeted CRISPR-Cas9 screening identifies core transcription factors controlling murine haemato-endothelial fate commitment
Michael Teske, Tobias Wertheimer, Stefan Butz, Pascale Zwicky, Izaskun Mallona, Svenja L Nopper, Christian Münz, Ulrich Elling, Christophe Lancrin, Burkhard Becher, Ana Rita Grosso, Tuncay Baubec, Nina Schmolka · 2025
Read Targeted CRISPR-Cas9 screening identifies core transcription factors controlling murine haemato-endothelial fate commitment by Michael Teske, Tobias Wertheimer, Stefan Butz, Pascale Zwicky, Izaskun Mallona, Svenja L Nopper, Christian Münz, Ulrich Elling, Christophe Lancrin, Burkhard Becher, Ana Rita Grosso, Tuncay Baubec, Nina Schmolka (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12738756.txt.
Description
Michael Teske and co-authors hunt for genes that steer early blood growth in this 2025 study. In a growing embryo, blood first forms in the yolk sac from a shared layer that can become blood or vessel lining. The team used mouse stem cells that mimic this early step in the lab.
They knocked out genes one by one with a gene-editing tool, focusing on genes that control other genes or shape DNA packaging. Five factors stood out as needed for the shared layer to form. Cells missing these factors leaned toward other fates. Gene activity checks showed what each factor changed. The work maps paths that may aid future blood stem cell work.
Catalog record
| CREATOR | Michael Teske, Tobias Wertheimer, Stefan Butz, Pascale Zwicky, Izaskun Mallona, Svenja L Nopper, Christian Münz, Ulrich Elling, Christophe Lancrin, Burkhard Becher, Ana Rita Grosso, Tuncay Baubec, Nina Schmolka |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 70 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |