Improved Detection Sensitivity of Spring Viremia of Carp Virus by Substituting a Two-Step with a One-Step Nested Reverse Transcription Polymerase Chain Reaction Method
Ji-Yoon Park, In-Joo Shin, Hyunwoo Kim, Eun Sup Lee, Euna Choi, Hyoung Jun Kim, Se Ryun Kwon · 2025
Read Improved Detection Sensitivity of Spring Viremia of Carp Virus by Substituting a Two-Step with a One-Step Nested Reverse Transcription Polymerase Chain Reaction Method by Ji-Yoon Park, In-Joo Shin, Hyunwoo Kim, Eun Sup Lee, Euna Choi, Hyoung Jun Kim, Se Ryun Kwon (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12734977.txt.
Description
Park and six co-authors improve detection of spring viremia of carp virus with a one-step nested test, in a study from twenty twenty-five. The contagious fish disease causes bleeding, swelling, and high juvenile deaths. The recommended two-step test is slow and contamination-prone. Their one-step version merges early stages into a single tube.
Sensitivity improves a thousand-fold over the two-step method. Detection reaches far lower virus dilutions reliably. Fish tissue detection rates rise from about eighty-four to ninety-two percent. Fewer handling steps cut time and contamination risk. Adoption in international guidelines is proposed by the authors.
Catalog record
| CREATOR | Ji-Yoon Park, In-Joo Shin, Hyunwoo Kim, Eun Sup Lee, Euna Choi, Hyoung Jun Kim, Se Ryun Kwon |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 18 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |