Comparison of Cultures and 16S/18S Amplicon-Based Microbiome Analyses for Diagnosing Nosocomial Pneumonia in Patients Admitted to the Intensive Care Unit—An Exploratory Study
Dennis Back Holmgaard, Lars Nebrich, Bülent Uslu, Christian Højte Schouw, Rimtas Dargis, Henrik Planck Pedersen, Kurt Fuursted, Henrik Vedel Nielsen, Jens Jørgen Christensen, Xiaohui Chen Nielsen, Lone Musaeus Poulsen, Alessandro Russo · 2025
Read Comparison of Cultures and 16S/18S Amplicon-Based Microbiome Analyses for Diagnosing Nosocomial Pneumonia in Patients Admitted to the Intensive Care Unit—An Exploratory Study by Dennis Back Holmgaard, Lars Nebrich, Bülent Uslu, Christian Højte Schouw, Rimtas Dargis, Henrik Planck Pedersen, Kurt Fuursted, Henrik Vedel Nielsen, Jens Jørgen Christensen, Xiaohui Chen Nielsen, Lone Musaeus Poulsen, Alessandro Russo (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12731414.txt.
Description
Pneumonia caught in intensive care is hard to pin down: prior antibiotics muddy cultures, and several microbes may share blame. Dennis Back Holmgaard and co-authors compared in 2025 two routes to naming the culprit: culturing samples from 23 ICU patients, and gene-based profiling of the same samples.
The two sampling routes mostly agreed with each other under both methods. Profiling saw a wider world: it caught organisms culture missed entirely, including a dog-associated Pasteurella and the Whipple's disease bacterium. In about a third of samples, both methods named the same pathogen. Culture still earned its place as the cheaper, targeted test. The authors present sequencing as a second pair of eyes for ICU pneumonia, best at exposing what growth-based methods overlook.
Catalog record
| CREATOR | Dennis Back Holmgaard, Lars Nebrich, Bülent Uslu, Christian Højte Schouw, Rimtas Dargis, Henrik Planck Pedersen, Kurt Fuursted, Henrik Vedel Nielsen, Jens Jørgen Christensen, Xiaohui Chen Nielsen, Lone Musaeus Poulsen, Alessandro Russo |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 30 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |