High-Efficiency Cryopreservation of Silver Pomfret Sperm: Protocol Development and Cryodamage Assessment

Man Zhang, Yijun Jiang, Yubei Qiu, Zukang Feng, Xianglong Chen, Chongyang Wang, Yuanbo Li, Qinqin Dai, Jiabao Hu, Xiaojun Yan, Yajun Wang · 2025

Read High-Efficiency Cryopreservation of Silver Pomfret Sperm: Protocol Development and Cryodamage Assessment by Man Zhang, Yijun Jiang, Yubei Qiu, Zukang Feng, Xianglong Chen, Chongyang Wang, Yuanbo Li, Qinqin Dai, Jiabao Hu, Xiaojun Yan, Yajun Wang (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12729387.txt.

Description

Eleven researchers report a study from two thousand twenty-five on freezing fish sperm for later use. The fish is the silver pomfret, a food fish of the Indo-West Pacific whose wild numbers have fallen under habitat damage and overfishing. Frozen sperm banks could support breeding long after capture.

The team tuned each step of the freezing process: the fluid the sperm sit in, the protective agent, dilution, cooling height, and thawing heat. One fluid gave the best post-thaw movement, above ninety-five percent. They also checked the thawed sperm for enzyme activity and fine structure to judge freezing damage. The result is a full protocol for this species.

Catalog record

CREATORMan Zhang, Yijun Jiang, Yubei Qiu, Zukang Feng, Xianglong Chen, Chongyang Wang, Yuanbo Li, Qinqin Dai, Jiabao Hu, Xiaojun Yan, Yajun Wang
YEAR2025
COLLECTIONPAPERS
FORMATTXT
FILE SIZE37 KB
VERSION1.0
LICENSECC BY 4.0
RIGHTSCC BY
SOURCEEurope PMC

Source: https://europepmc.org/articles/PMC12729387

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