Isothermal and protein-free cascade catalytic hairpin assembly induced-DNAzyme sensing strategy for sensitive miRNA analysis
Yuepeng Zhang, Yuhua Sun, Xueshan Gong, Shuang Zhao, Weiwei Cao, Hongbo Wang, Hao Wang, Xi Zhang, Changwei Du, Zhiguo Chen, Lu Deng · 2025
Read Isothermal and protein-free cascade catalytic hairpin assembly induced-DNAzyme sensing strategy for sensitive miRNA analysis by Yuepeng Zhang, Yuhua Sun, Xueshan Gong, Shuang Zhao, Weiwei Cao, Hongbo Wang, Hao Wang, Xi Zhang, Changwei Du, Zhiguo Chen, Lu Deng (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12728169.txt.
Description
Yuepeng Zhang and co-authors build a sensitive test for a cancer-linked microRNA in a 2025 paper. MicroRNAs are tiny RNA strands whose levels can flag tumors early. MicroRNA-21 is a known marker, but standard tests need strict temperatures or protein enzymes. Their test runs at one steady temperature with no proteins at all, in a single pot.
The target strand starts a chain of hairpin DNA pieces opening each other in turn. That chain frees a DNAzyme, a DNA strand that cuts other strands. With magnesium present, the DNAzyme clips glowing reporter pieces again and again, building a bright signal. The test spotted the target down to a very low level in about an hour. It could tell apart a single wrong letter in the strand. Serum samples gave robust results too.
Catalog record
| CREATOR | Yuepeng Zhang, Yuhua Sun, Xueshan Gong, Shuang Zhao, Weiwei Cao, Hongbo Wang, Hao Wang, Xi Zhang, Changwei Du, Zhiguo Chen, Lu Deng |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 29 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |