Establishment and application of a quadruple RT-qPCR method for simultaneous detection of porcine enteric coronaviruses
Caiwang Ye, Jingru Xu, Sisi Fan, Fangting Chen, Shuqi Qiu, Huachun Pan, Shumin Yang, Yuqi Li, Yuting Liao, Weiye Lin, Xiaoziyi Xiao, Xuejin Li, Yuxi Xue, Yali Kang, Yubin Zhuo, Lingshan Huang, Xiaoping Wu, Ailing Dai, Nana Yan, Kewei Fan · 2025
Read Establishment and application of a quadruple RT-qPCR method for simultaneous detection of porcine enteric coronaviruses by Caiwang Ye, Jingru Xu, Sisi Fan, Fangting Chen, Shuqi Qiu, Huachun Pan, Shumin Yang, Yuqi Li, Yuting Liao, Weiye Lin, Xiaoziyi Xiao, Xuejin Li, Yuxi Xue, Yali Kang, Yubin Zhuo, Lingshan Huang, Xiaoping Wu, Ailing Dai, Nana Yan, Kewei Fan (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12727581.txt.
Description
Caiwang Ye and many colleagues published a 2025 study establishing a laboratory test that detects four pig coronaviruses at once. These porcine enteric coronaviruses cause severe diarrhea in piglets and heavy losses for the pig industry. The four include the viruses behind epidemic diarrhea and transmissible gastroenteritis.
Because the four infections look nearly identical in sick animals, telling them apart requires a molecular test. The team's quadruple RT-qPCR assay runs four detections in a single reaction, each tagged so the viruses can be distinguished. They describe building the method and applying it to real samples. A one-tube test lets farms and veterinary laboratories identify which virus is present quickly and plan control measures.
Catalog record
| CREATOR | Caiwang Ye, Jingru Xu, Sisi Fan, Fangting Chen, Shuqi Qiu, Huachun Pan, Shumin Yang, Yuqi Li, Yuting Liao, Weiye Lin, Xiaoziyi Xiao, Xuejin Li, Yuxi Xue, Yali Kang, Yubin Zhuo, Lingshan Huang, Xiaoping Wu, Ailing Dai, Nana Yan, Kewei Fan |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 26 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |