Identification of strong constitutive promoters in Burkholderia stagnalis TBRC 18363 for activating natural product production in Gram-negative bacteria
Chayaphat Wongsombat, Wuttichai Mhuantong, Jindaporn Kongsee, Thapanee Pruksatrakul, Kitlada Srichomthong, Piyanat Charoenyingcharoen, Umaporn Uawisetwathana, Pattaraporn Yukphan, Vanicha Vichai, Dominik Pistorius, Kathrin Buntin, Aiyada Aroonsri · 2025
Read Identification of strong constitutive promoters in Burkholderia stagnalis TBRC 18363 for activating natural product production in Gram-negative bacteria by Chayaphat Wongsombat, Wuttichai Mhuantong, Jindaporn Kongsee, Thapanee Pruksatrakul, Kitlada Srichomthong, Piyanat Charoenyingcharoen, Umaporn Uawisetwathana, Pattaraporn Yukphan, Vanicha Vichai, Dominik Pistorius, Kathrin Buntin, Aiyada Aroonsri (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12718240.txt.
Description
Wongsombat and colleagues published in twenty twenty-five a search for strong gene switches in a Burkholderia bacterium. Some bacteria make useful natural products, but their genes are often silent. A strong promoter, a DNA switch that stays on, can wake those genes. The team read the genome of one strain and tracked which genes ran hottest. They guessed the DNA just before those genes held strong switches.
They tested twenty-six candidate switches in two other bacteria. Two switches beat the rest in both hosts. Swapping them into product gene clusters raised yields of two compounds. One product blocks a cell signal protein of medical interest. The switches could help unlock hidden products in many Gram-negative bacteria.
Catalog record
| CREATOR | Chayaphat Wongsombat, Wuttichai Mhuantong, Jindaporn Kongsee, Thapanee Pruksatrakul, Kitlada Srichomthong, Piyanat Charoenyingcharoen, Umaporn Uawisetwathana, Pattaraporn Yukphan, Vanicha Vichai, Dominik Pistorius, Kathrin Buntin, Aiyada Aroonsri |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 35 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |