Applying 3D correlative structured illumination microscopy and X-ray tomography to characterise herpes simplex virus-1 morphogenesis
Kamal L Nahas, Viv Connor, Kaveesha J Wijesinghe, Henry G Barrow, Ian M Dobbie, Maria Harkiolaki, Stephen C Graham, Colin M Crump, Laura Ruth Delgui, Kamal L Nahas, Viv Connor, Kaveesha J Wijesinghe, Henry G Barrow, Ian M Dobbie, Maria Harkiolaki, Stephen C Graham, Colin M Crump · 2025
Read Applying 3D correlative structured illumination microscopy and X-ray tomography to characterise herpes simplex virus-1 morphogenesis by Kamal L Nahas, Viv Connor, Kaveesha J Wijesinghe, Henry G Barrow, Ian M Dobbie, Maria Harkiolaki, Stephen C Graham, Colin M Crump, Laura Ruth Delgui, Kamal L Nahas, Viv Connor, Kaveesha J Wijesinghe, Henry G Barrow, Ian M Dobbie, Maria Harkiolaki, Stephen C Graham, Colin M Crump (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12716837.txt.
Description
Kamal Nahas and co-authors image in 2025 how the herpes simplex virus builds itself inside infected cells. Many viral genes help in the assembly, but which matter most at each stage was murky. The team paired two 3D imaging methods on the same cells: one that tags viral parts with glowing labels, and one that maps the cell's inner structure with X-rays.
Nine mutant viruses, each missing one protein, were compared with the normal virus. Five proteins were ranked by how much their loss slowed the virus's exit from the nucleus. Other proteins guided the shell's delivery and final wrapping, with stalled wrapping forms never seen before.
Catalog record
| CREATOR | Kamal L Nahas, Viv Connor, Kaveesha J Wijesinghe, Henry G Barrow, Ian M Dobbie, Maria Harkiolaki, Stephen C Graham, Colin M Crump, Laura Ruth Delgui, Kamal L Nahas, Viv Connor, Kaveesha J Wijesinghe, Henry G Barrow, Ian M Dobbie, Maria Harkiolaki, Stephen C Graham, Colin M Crump |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 135 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |