A green lifetime biosensor for calcium that remains bright over its full dynamic range
Franka H van der Linden, Stephen C Thornquist, Rick M ter Beek, Jelle Y Huijts, Mark A Hink, Theodorus W J Gadella, Gaby Maimon, Joachim Goedhart, Alejandro San Martín, Franka H van der Linden, Stephen C Thornquist, Rick M ter Beek, Jelle Y Huijts, Mark Hink, Theodorus Gadella, Gaby Maimon, Joachim · 2025
Read A green lifetime biosensor for calcium that remains bright over its full dynamic range by Franka H van der Linden, Stephen C Thornquist, Rick M ter Beek, Jelle Y Huijts, Mark A Hink, Theodorus W J Gadella, Gaby Maimon, Joachim Goedhart, Alejandro San Martín, Franka H van der Linden, Stephen C Thornquist, Rick M ter Beek, Jelle Y Huijts, Mark Hink, Theodorus Gadella, Gaby Maimon, Joachim (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12716836.txt.
Description
Franka van der Linden and co-authors present a new calcium sensor for live-cell imaging in this 2025 paper. Sensors that glow when calcium rises are workhorses of cell biology, but most switch between a bright state and a dim one. The dim state gives a weak signal, which hurts precise measurement.
While tuning a sensor of a different color, the team found a green version that stays bright in both states. Calcium still changes how long its glow lasts, by more than a billionth of a second, so lifetime imaging can read the level. The sensor, named G-Ca-FLITS, is about as bright as widely used sensors and barely reacts to acidity. With it, the team tracked calcium in cell compartments, saw uneven bursts inside mitochondria, and tested lifetime imaging in fruit fly brains.
Catalog record
| CREATOR | Franka H van der Linden, Stephen C Thornquist, Rick M ter Beek, Jelle Y Huijts, Mark A Hink, Theodorus W J Gadella, Gaby Maimon, Joachim Goedhart, Alejandro San Martín, Franka H van der Linden, Stephen C Thornquist, Rick M ter Beek, Jelle Y Huijts, Mark Hink, Theodorus Gadella, Gaby Maimon, Joachim |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 134 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |