Anticodon-edited tRNA enables translational readthrough of COL4A5 premature termination codons
Kohei Omachi, Joseph J. Porter, John D. Lueck, Jeffrey H. Miner · 2025
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Description
Alport syndrome damages the kidney's filter when a collagen gene carries a stop signal that cuts the protein short. Kohei Omachi, Joseph Porter, John Lueck, and Jeffrey Miner tested a rescue idea in 2025: edited transfer RNAs that read through the stop signal so the full protein gets built. They tried it on four patient variants of the COL4A5 gene in lab cells, using a light-producing tag that only glows if the protein is completed.
The edited RNAs restored the glow for all four variants, and protein tests confirmed full-length chains. The rescued protein also joined its two partner chains into the normal three-chain unit. The authors present the approach as a promising path for nonsense-variant disease.
Catalog record
| CREATOR | Kohei Omachi, Joseph J. Porter, John D. Lueck, Jeffrey H. Miner |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |