ST-FFPE-mIF: integrating spatial transcriptomics and multiplex immunofluorescence in formalin-fixed paraffin-embedded tissues using Stereo-seq
Xue Zhang, Meng Zhang, Yuan Xu, Yangyang Song, Xingfeng Yang, Yanan Wu, Xuelin Zhao, Dongsheng Ran, Xin Liu, Huaqiang Huang, Wenxiao Lei, Hongyan Li, Yongfen Zhang, Feng Xi, Guibo Li, Xing Liu, Luohao Xu, Ao Chen, Sha Liao, Jiajun Zhang · 2025
Read ST-FFPE-mIF: integrating spatial transcriptomics and multiplex immunofluorescence in formalin-fixed paraffin-embedded tissues using Stereo-seq by Xue Zhang, Meng Zhang, Yuan Xu, Yangyang Song, Xingfeng Yang, Yanan Wu, Xuelin Zhao, Dongsheng Ran, Xin Liu, Huaqiang Huang, Wenxiao Lei, Hongyan Li, Yongfen Zhang, Feng Xi, Guibo Li, Xing Liu, Luohao Xu, Ao Chen, Sha Liao, Jiajun Zhang (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12709731.txt.
Description
Stored tissue samples can now yield both gene maps and protein stains in the same slice, thanks to a 2025 protocol by Zhang and co-authors. Hospitals keep vast archives of wax-embedded tissue, but older gene mapping methods read genes alone. The new protocol adds an eight-color stain step to a spatial gene method for such samples.
Stains mark cell types, while gene data show activity in place. Cell borders are drawn from nucleus or membrane stains, giving single-cell detail. Tests on cancer samples show the paired method works well. Labs can use the protocol to link what cells are with what they do, all in one archived slice. That pairing may sharpen cancer studies that rely on stored samples.
Catalog record
| CREATOR | Xue Zhang, Meng Zhang, Yuan Xu, Yangyang Song, Xingfeng Yang, Yanan Wu, Xuelin Zhao, Dongsheng Ran, Xin Liu, Huaqiang Huang, Wenxiao Lei, Hongyan Li, Yongfen Zhang, Feng Xi, Guibo Li, Xing Liu, Luohao Xu, Ao Chen, Sha Liao, Jiajun Zhang |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 46 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |