Protocol for capturing the RNA-binding proteome from plants using orthogonal organic phase separation
Victor A Sánchez-Camargo, Gertjan Kramer, Harrold A van den Burg · 2025
Read Protocol for capturing the RNA-binding proteome from plants using orthogonal organic phase separation by Victor A Sánchez-Camargo, Gertjan Kramer, Harrold A van den Burg (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12702337.txt.
Description
A peer-reviewed research article from Europe PMC (2025). SummaryRNA-binding proteins (RBPs) regulate many processes related to RNA biogenesis, function, localization, and degradation. Here, we present a protocol for the en masse isolation of RBPs crosslinked to RNA and provide a strategy for subsequent proteomics analysis. We describe the steps for in vivo UV crosslinking of RNA-protein complexes, tissue lysis, fractionation, and purification of crosslinked RNA-protein adducts using organic solvents.
Catalog record
| CREATOR | Victor A Sánchez-Camargo, Gertjan Kramer, Harrold A van den Burg |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 109 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |