An optimised whole blood interleukin‐2 release assay is more sensitive than interferon‐γ ELISpot for detecting and quantifying gluten‐specific CD4+ T‐cell responses in coeliac disease
Olivia G Moscatelli, Amy K Russell, Lee M Henneken, Melinda Y Hardy, Jason A Tye‐Din · 2025
Read An optimised whole blood interleukin‐2 release assay is more sensitive than interferon‐γ ELISpot for detecting and quantifying gluten‐specific CD4+ T‐cell responses in coeliac disease by Olivia G Moscatelli, Amy K Russell, Lee M Henneken, Melinda Y Hardy, Jason A Tye‐Din (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12695692.txt.
Description
Olivia Moscatelli, Amy Russell, Lee Henneken, Melinda Hardy, and Jason Tye-Din compare two blood tests for the gluten-reactive T cells of coeliac disease. Finding these rare cells now needs a standard test plus days of eating gluten to raise their numbers. A simpler, sharper test would ease both study and care.
Ten treated adults were tested before and about a week after a single gluten dose. Three readouts ran side by side: blood serum, a whole-blood test, and the standard cell spot test. The whole-blood test, tuned to catch a key immune signal, proved the most sensitive. It also measured the response size more cleanly. Peptide and blocking tests checked that the signal was truly gluten-specific.
Catalog record
| CREATOR | Olivia G Moscatelli, Amy K Russell, Lee M Henneken, Melinda Y Hardy, Jason A Tye‐Din |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 39 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |