Optimizing Small RNA Sequencing for Salivary Biomarker Identification: A Comparative Study of Library Preparation Protocols
Ulrike Kegler, Nathalie Ropek, Manuela Hofner, Silvia Schönthaler, Klemens Vierlinger, Christa Nöhammer, Erik AC Wiemer · 2025
Read Optimizing Small RNA Sequencing for Salivary Biomarker Identification: A Comparative Study of Library Preparation Protocols by Ulrike Kegler, Nathalie Ropek, Manuela Hofner, Silvia Schönthaler, Klemens Vierlinger, Christa Nöhammer, Erik AC Wiemer (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12692187.txt.
Description
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and hold significant potential as biomarkers. Saliva, a non-invasive and easily accessible biofluid, offers a promising alternative to blood for miRNA-based diagnostics. However, miRNA profiling by next-generation sequencing (NGS) is highly influenced by library preparation protocol, which can introduce detection and quantification biases.
Catalog record
| CREATOR | Ulrike Kegler, Nathalie Ropek, Manuela Hofner, Silvia Schönthaler, Klemens Vierlinger, Christa Nöhammer, Erik AC Wiemer |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 46 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |