Search-and-remove genome editing allows selection of cells by DNA sequence
Luise Fast, Madina Omar, Philipp Kanis, Theresa Schaffer, Deepika Chowdhury, Eva Rakava, Svante Pääbo, Stephan Riesenberg · 2025
Read Search-and-remove genome editing allows selection of cells by DNA sequence by Luise Fast, Madina Omar, Philipp Kanis, Theresa Schaffer, Deepika Chowdhury, Eva Rakava, Svante Pääbo, Stephan Riesenberg (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12689631.txt.
Description
Fast and colleagues published in twenty twenty-five a genome editing method that selects edited cells without marker genes. Editors usually add drug resistance or glow markers to find edited cells. Those markers can disturb cells and fail for single-letter edits. Their method, SNIPE, selects by the DNA sequence itself. It enriches precisely edited cells about sevenfold on median. The team validated it across forty-two edits in varied cell types and species.
They used it to restore ancestral gene letters seen in Neandertals and Denisovans. Modern humans carry changed versions at those sites. The method also killed cultured tumor cells with abnormal genotypes. It repaired a disease-causing mutation carried on one gene copy. Marker-free selection widens what precision editing can attempt, the authors argue.
Catalog record
| CREATOR | Luise Fast, Madina Omar, Philipp Kanis, Theresa Schaffer, Deepika Chowdhury, Eva Rakava, Svante Pääbo, Stephan Riesenberg |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 53 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |