Label-free in-line characterization of immune cell culture using quantitative phase imaging
Caroline E. Serafini, Viswanath Gorti, Paloma Casteleiro Costa, Aaron D. Silva Trenkle, Bharat Kanwar, Bryan Wang, Brian Wicker, Linda E. Kippner, Isaac LeCompte, Rui Qi Chen, Benjamin Joffe, Ye Li, Annie C. Bowles-Welch, Jing Li, Christine E. Brown, Gabriel A. Kwong, Stephen Balakirsky, Krishnendu · 2025
Read Label-free in-line characterization of immune cell culture using quantitative phase imaging by Caroline E. Serafini, Viswanath Gorti, Paloma Casteleiro Costa, Aaron D. Silva Trenkle, Bharat Kanwar, Bryan Wang, Brian Wicker, Linda E. Kippner, Isaac LeCompte, Rui Qi Chen, Benjamin Joffe, Ye Li, Annie C. Bowles-Welch, Jing Li, Christine E. Brown, Gabriel A. Kwong, Stephen Balakirsky, Krishnendu (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12660828.txt.
Description
A large team of co-authors reports a new tool in a paper from two thousand twenty-five for watching immune cells as they grow. Cell therapies, such as T cell treatments, need close checks during culture. Sampling the cells can break sterility and adds cost, and weak checks lead to uneven batches.
The team built a small, low-cost imaging platform that reads cells without dyes or labels. The method, called quantitative phase imaging, works inside the growth vessel while culture runs. Image-based tests built by the team measured how many cells were alive and how active they were. The tests held up across cells from more than fifty donors.
Catalog record
| CREATOR | Caroline E. Serafini, Viswanath Gorti, Paloma Casteleiro Costa, Aaron D. Silva Trenkle, Bharat Kanwar, Bryan Wang, Brian Wicker, Linda E. Kippner, Isaac LeCompte, Rui Qi Chen, Benjamin Joffe, Ye Li, Annie C. Bowles-Welch, Jing Li, Christine E. Brown, Gabriel A. Kwong, Stephen Balakirsky, Krishnendu |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 63 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |