Engineering Streptomyces coelicolor for heterologous expression of the thiopeptide GE2270A—A cautionary tale
Francesco Del Carratore, Erik K R Hanko, Kamila Schmidt, Oksana Bilyk, Suhui Ye Huang, Marianna Iorio, Mercedes Pérez-Bonilla, Rosario Pérez-Redondo, Michelle Rudden, Emmanuele Severi, Arianna Tocchetti, Margherita Sosio, Emily J Johnson, Timothy Kirkwood, Dominic R Whittall, Alkisti Manousaki, Olga · 2025
Read Engineering Streptomyces coelicolor for heterologous expression of the thiopeptide GE2270A—A cautionary tale by Francesco Del Carratore, Erik K R Hanko, Kamila Schmidt, Oksana Bilyk, Suhui Ye Huang, Marianna Iorio, Mercedes Pérez-Bonilla, Rosario Pérez-Redondo, Michelle Rudden, Emmanuele Severi, Arianna Tocchetti, Margherita Sosio, Emily J Johnson, Timothy Kirkwood, Dominic R Whittall, Alkisti Manousaki, Olga (2025) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12309360.txt.
Description
Francesco Del Carratore and co-authors tell a cautionary tale about making an antibiotic in a borrowed host. The thiopeptide GE2270A comes from a bacterium that resists genetic tinkering, so the team moved its gene cluster into a cooperative model species, Streptomyces coelicolor, and tuned the design with data-driven engineering.
Extra copies of key genes lifted yields clearly. Yet the best strain still made twelve times less than the natural producer and fifty times less than another host species used in earlier work. Deep molecular profiling pointed to one gene's key role and mapped how the strains' chemistries diverged. The lesson: an easy host speeds design trials, but host choice caps the final yield.
Catalog record
| CREATOR | Francesco Del Carratore, Erik K R Hanko, Kamila Schmidt, Oksana Bilyk, Suhui Ye Huang, Marianna Iorio, Mercedes Pérez-Bonilla, Rosario Pérez-Redondo, Michelle Rudden, Emmanuele Severi, Arianna Tocchetti, Margherita Sosio, Emily J Johnson, Timothy Kirkwood, Dominic R Whittall, Alkisti Manousaki, Olga |
|---|---|
| YEAR | 2025 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 54 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |