Selection of reference genes for quantitative real-time PCR analysis in exogenous hormone-treated Lycoris aurea

Wei Zhao, Ying Tian, Yi Wang, Yun Wu, Ziming Ren, Kehu Li · 2024

Read Selection of reference genes for quantitative real-time PCR analysis in exogenous hormone-treated Lycoris aurea by Wei Zhao, Ying Tian, Yi Wang, Yun Wu, Ziming Ren, Kehu Li (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-12040745.txt.

Description

Wei Zhao and co-authors hunt for stable reference genes in a flowering bulb plant treated with growth hormones. The plant, Lycoris aurea from southern China, makes valued alkaloids used against viruses, tumors, and memory loss. Gene studies of its bulbs rely on a measuring method that needs steady reference genes to stay accurate, and hormone treatment can upset that steadiness.

The team picked seven candidate reference genes and measured each in bulbs exposed to seven hormone treatments over two days. Four statistical programs, each with its own logic, rated the candidates' stability. The work, published in 2024, aims to give later studies a firm base for comparing gene activity in this plant under hormone treatment. Sound references matter because a drifting yardstick can fake a change in every gene measured against it.

Catalog record

CREATORWei Zhao, Ying Tian, Yi Wang, Yun Wu, Ziming Ren, Kehu Li
YEAR2024
COLLECTIONPAPERS
FORMATTXT
FILE SIZE22 KB
VERSION1.0
LICENSECC BY 4.0
RIGHTSCC BY
SOURCEEurope PMC

Source: https://europepmc.org/articles/PMC12040745

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