Accounting for fast vs slow exchange in single molecule FRET experiments reveals hidden conformational states
Justin J. Miller, Upasana L. Mallimadugula, Maxwell I. Zimmerman, Melissa D. Stuchell-Brereton, Andrea Soranno, Gregory R. Bowman · 2024
Read Accounting for fast vs slow exchange in single molecule FRET experiments reveals hidden conformational states by Justin J. Miller, Upasana L. Mallimadugula, Maxwell I. Zimmerman, Melissa D. Stuchell-Brereton, Andrea Soranno, Gregory R. Bowman (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11886876.txt.
Description
Justin Miller and co-authors fix a blind spot in a lab method that watches single proteins flex, in a 2024 paper. The method, smFRET, reads energy hops between dyes on one protein at a time. Paired with simulations, it could yield full atom-level models of protein shapes. The two views often clash, though, which blocks that goal.
The snag is time: each reading blurs all the shapes a protein takes while the dye glows. The team folds that blur into the math, using motion maps from simulations to say which states merge. Fit between lab and computer then rose a lot, for stiff, loose, and fully disordered proteins alike. The fit also exposed states the lab view had missed. Left-over gaps point to where new simulations should hunt.
Catalog record
| CREATOR | Justin J. Miller, Upasana L. Mallimadugula, Maxwell I. Zimmerman, Melissa D. Stuchell-Brereton, Andrea Soranno, Gregory R. Bowman |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 36 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |