‘Iterative Bleaching Extends Multiplexity’ facilitates simultaneous identification of all major retinal cell types
Aanandita A. Kothurkar, Gregory S. Patient, Nicole C. L. Noel, Aleksandra M. Krzywańska, Brittany J. Carr, Colin J. Chu, Ryan B. MacDonald · 2024
Read ‘Iterative Bleaching Extends Multiplexity’ facilitates simultaneous identification of all major retinal cell types by Aanandita A. Kothurkar, Gregory S. Patient, Nicole C. L. Noel, Aleksandra M. Krzywańska, Brittany J. Carr, Colin J. Chu, Ryan B. MacDonald (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11827602.txt.
Description
Kothurkar and co-authors adapted a many-label imaging method to the zebrafish retina, in a study published in 2024. To understand a tissue, researchers need to see all its cell types at once. Standard staining can only show a few labels together, because the colors overlap. The method they used labels a sample in rounds, bleaching each round's glow before the next. Eleven antibodies were chained this way on one piece of tissue.
The full set lit up every major cell type of the retina at once. The team also made the method work alongside fish lines that glow on their own in set cells. Retinas are a favorite model for brain wiring and eye disease. A complete cellular map of one sample saves animals and aligns data perfectly. The adaptation opens the same trick to other fish tissues.
Catalog record
| CREATOR | Aanandita A. Kothurkar, Gregory S. Patient, Nicole C. L. Noel, Aleksandra M. Krzywańska, Brittany J. Carr, Colin J. Chu, Ryan B. MacDonald |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 56 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |