Analyses of translation factors Dbp1 and Ded1 reveal the cellular response to heat stress to be separable from stress granule formation
Naohiro Kuwayama, Emily Nicole Powers, Matej Siketanc, Camila Ines Sousa, Kendra Reynaud, Marko Jovanovic, Maria Hondele, Nicholas Thomas Ingolia, Gloria Ann Brar · 2024
Read Analyses of translation factors Dbp1 and Ded1 reveal the cellular response to heat stress to be separable from stress granule formation by Naohiro Kuwayama, Emily Nicole Powers, Matej Siketanc, Camila Ines Sousa, Kendra Reynaud, Marko Jovanovic, Maria Hondele, Nicholas Thomas Ingolia, Gloria Ann Brar (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11759133.txt.
Description
Naohiro Kuwayama and eight co-authors use two yeast translation factors to show that a cell's heat response is not the same thing as forming stress granules. Ded1 and its relative Dbp1 are helicases that start protein synthesis. Under starvation their levels swap, but swapping the genes shows Dbp1 cannot do Ded1's everyday job.
Matched-level experiments found Ded1 the stronger activator, especially for messenger RNAs with structured leaders. Heat normally shuts down those transcripts and halts growth; cells running on Dbp1 failed to halt. Surprisingly, granule formation and bulk translation shutdown stayed intact in those cells. The failure traced to an eleven-amino-acid stretch that disables Ded1 when heated. Granules, the authors conclude, are a separable module of the stress response.
Catalog record
| CREATOR | Naohiro Kuwayama, Emily Nicole Powers, Matej Siketanc, Camila Ines Sousa, Kendra Reynaud, Marko Jovanovic, Maria Hondele, Nicholas Thomas Ingolia, Gloria Ann Brar |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 78 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |