Modulation of TCR stimulation and pifithrin-α improves the genomic safety profile of CRISPR-engineered human T cells
Laurenz T. Ursch, Jule S. Müschen, Julia Ritter, Julia Klermund, Bettina E. Bernard, Saskia Kolb, Linda Warmuth, Geoffroy Andrieux, Gregor Miller, Marina Jiménez-Muñoz, Fabian J. Theis, Melanie Boerries, Dirk H. Busch, Toni Cathomen, Kathrin Schumann · 2024
Read Modulation of TCR stimulation and pifithrin-α improves the genomic safety profile of CRISPR-engineered human T cells by Laurenz T. Ursch, Jule S. Müschen, Julia Ritter, Julia Klermund, Bettina E. Bernard, Saskia Kolb, Linda Warmuth, Geoffroy Andrieux, Gregor Miller, Marina Jiménez-Muñoz, Fabian J. Theis, Melanie Boerries, Dirk H. Busch, Toni Cathomen, Kathrin Schumann (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11722128.txt.
Description
Laurenz Ursch and a large team of co-authors study the genomic safety of CRISPR-edited T cells in two thousand twenty-four. Such edited cells lead new cancer treatments, but CRISPR can cause large deletions and chromosome errors. Past safety work focused on the editing tools, less on the T cells themselves.
The team found that strong T cell stimulation and fast growth correlate with larger deletions. Editing quiet, non-activated cells cut that risk but lowered knockout rates. Adding a small molecule, pifithrin-alpha, also limited deletions, translocations, and chromosome count errors. It did so without hurting the cells' function, including in CAR T cells. Both strategies are easy to adopt, the authors say.
Catalog record
| CREATOR | Laurenz T. Ursch, Jule S. Müschen, Julia Ritter, Julia Klermund, Bettina E. Bernard, Saskia Kolb, Linda Warmuth, Geoffroy Andrieux, Gregor Miller, Marina Jiménez-Muñoz, Fabian J. Theis, Melanie Boerries, Dirk H. Busch, Toni Cathomen, Kathrin Schumann |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 80 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |