Deep phosphoproteomics of Klebsiella pneumoniae reveals HipA-mediated tolerance to ciprofloxacin
Payal Nashier, Isabell Samp, Marvin Adler, Fiona Ebner, Lisa Thai Lê, Marc Göppel, Carsten Jers, Ivan Mijakovic, Sandra Schwarz, Boris Macek, D. Scott Samuels, Jose Luis Balcazar, D. Scott Samuels, Jose Luis Balcazar, D. Scott Samuels, Jose Luis Balcazar · 2024
Read Deep phosphoproteomics of Klebsiella pneumoniae reveals HipA-mediated tolerance to ciprofloxacin by Payal Nashier, Isabell Samp, Marvin Adler, Fiona Ebner, Lisa Thai Lê, Marc Göppel, Carsten Jers, Ivan Mijakovic, Sandra Schwarz, Boris Macek, D. Scott Samuels, Jose Luis Balcazar, D. Scott Samuels, Jose Luis Balcazar, D. Scott Samuels, Jose Luis Balcazar (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11717353.txt.
Description
Payal Nashier and colleagues map the phosphorylation landscape of Klebsiella pneumoniae, a major hospital pathogen resistant to antibiotics, and expose a kinase that helps it tolerate treatment. How this bacterium is run after its proteins are made is poorly known. The team ran a full phosphoproteome analysis on HipA, a serine-threonine kinase known from Escherichia coli for making cells withstand antibiotics.
Making too much Klebsiella HipA was toxic both to Klebsiella itself and to Escherichia coli, and the harm could be undone by making more of its antitoxin partner. HipA overproduction raised tolerance to ciprofloxacin, a widely used antibiotic against this pathogen. Proteome and phosphoproteome checks, with and without the drug, showed the kinase was active, including self-phosphorylation at one serine. The Klebsiella kinase also shares many substrates with its Escherichia coli partner.
Catalog record
| CREATOR | Payal Nashier, Isabell Samp, Marvin Adler, Fiona Ebner, Lisa Thai Lê, Marc Göppel, Carsten Jers, Ivan Mijakovic, Sandra Schwarz, Boris Macek, D. Scott Samuels, Jose Luis Balcazar, D. Scott Samuels, Jose Luis Balcazar, D. Scott Samuels, Jose Luis Balcazar |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 78 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |