Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures
Mariana Rebocho da Costa, Ana Ribeiro, Raquel Quitéria, Dalila Neves-Silva, Leonor Saúde · 2024
Read Protocol for whole-mount preparation, clearing, and visualization of the adult zebrafish spinal cord structures by Mariana Rebocho da Costa, Ana Ribeiro, Raquel Quitéria, Dalila Neves-Silva, Leonor Saúde (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11683226.txt.
Description
Mariana Rebocho da Costa and colleagues present a laboratory protocol for preparing, clearing, and visualising the adult zebrafish spinal cord whole. Their 2024 protocol supports research into vascular repair after spinal cord injury, a neglected topic in zebrafish work.
The protocol details extracting and dissecting whole spinal cords from adult fish. Optimised staining and clearing steps then make structures such as the vascular network visible in three dimensions. After clearing, samples can be reversed for further uses including gelatin embedding, thin sectioning, and additional staining. Full execution details are given step by step. The authors point readers to a related research article for complete usage context and examples.
Catalog record
| CREATOR | Mariana Rebocho da Costa, Ana Ribeiro, Raquel Quitéria, Dalila Neves-Silva, Leonor Saúde |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 105 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |