Development, Optimization, and Validation of a Quantitative PCR Assay for Borrelia burgdorferi Detection in Tick, Wildlife, and Human Samples
Julie Lewis, Vett K Lloyd, Gilles A Robichaud, Ana Domingos · 2024
Read Development, Optimization, and Validation of a Quantitative PCR Assay for Borrelia burgdorferi Detection in Tick, Wildlife, and Human Samples by Julie Lewis, Vett K Lloyd, Gilles A Robichaud, Ana Domingos (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11679815.txt.
Description
Julie Lewis, Vett Lloyd, Gilles Robichaud, and Ana Domingos published a 2024 study on a sharper test for the Lyme disease bacterium. The bacterium, Borrelia burgdorferi, spreads by tick bites. Finding it in ticks, wildlife, and people is key to tracking the disease.
Their test is a quantitative PCR assay that targets two of the bacterium's genes, ospA and flaB. On known tick samples, it beat the older nested PCR on speed, sensitivity, and accuracy. Wildlife tissue is a harder test bed, since the bacterium hides at low levels there. The assay held up on those samples too. A trusted test helps labs map where Lyme risk is growing.
Catalog record
| CREATOR | Julie Lewis, Vett K Lloyd, Gilles A Robichaud, Ana Domingos |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 76 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |