A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of Chrysodeixis includens and Rachiplusia nu
Guilherme A. Gotardi, Natália R. F. Batista, Tamylin Kaori Ishizuka, Luiz H. Marques, Mário H. Dal Pogetto, Amit Sethi, Mark L. Dahmer, Timothy Nowatzki · 2024
Read A Novel Polymerase Chain Reaction (PCR)-Based Method for the Rapid Identification of Chrysodeixis includens and Rachiplusia nu by Guilherme A. Gotardi, Natália R. F. Batista, Tamylin Kaori Ishizuka, Luiz H. Marques, Mário H. Dal Pogetto, Amit Sethi, Mark L. Dahmer, Timothy Nowatzki (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11679590.txt.
Description
Guilherme Gotardi and seven co-authors develop a fast PCR test to tell apart two look-alike moth pests, in work from 2024. Larvae of Chrysodeixis includens and Rachiplusia nu are hard to separate by eye, yet the species differ in how they respond to insecticides and Bt crops. Sequencing can split them, but it is slow and costly.
After sequencing part of a mitochondrial gene, the team built species-specific primers around the gaps they found. One shared reverse primer and two forward primers give fragments of distinct lengths for each species. Bands on a simple gel then name the species at once. Tests showed the primers to be specific, accurate and reliable for routine identification.
Catalog record
| CREATOR | Guilherme A. Gotardi, Natália R. F. Batista, Tamylin Kaori Ishizuka, Luiz H. Marques, Mário H. Dal Pogetto, Amit Sethi, Mark L. Dahmer, Timothy Nowatzki |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 13 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |