Non-Invasive Nanometer Resolution Assessment of Cell–Soft Hydrogel System Mechanical Properties by Scanning Ion Conductance Microscopy
Tatiana N Tikhonova, Anastasia V Barkovaya, Yuri M Efremov, Vugara V Mamed-Nabizade, Vasilii S Kolmogorov, Peter S Timashev, Nikolay N Sysoev, Victor V Fadeev, Petr V Gorelkin, Lihi Adler-Abramovich, Alexander S Erofeev, Evgeny A Shirshin, José Luis Toca-Herrera · 2024
Read Non-Invasive Nanometer Resolution Assessment of Cell–Soft Hydrogel System Mechanical Properties by Scanning Ion Conductance Microscopy by Tatiana N Tikhonova, Anastasia V Barkovaya, Yuri M Efremov, Vugara V Mamed-Nabizade, Vasilii S Kolmogorov, Peter S Timashev, Nikolay N Sysoev, Victor V Fadeev, Petr V Gorelkin, Lihi Adler-Abramovich, Alexander S Erofeev, Evgeny A Shirshin, José Luis Toca-Herrera (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11678895.txt.
Description
Tatiana Tikhonova and co-authors measured this 2024 softness of living cancer cells without touching them hard. Cells grown on soft gels, the stuff of tissue scaffolds, change their own stiffness, but standard poking tools are invasive and coarse. The team used scanning ion conductance microscopy, which senses surfaces from ion currents, to map breast cancer cells growing on a self-assembled peptide gel at nanometer resolution.
The stiffness maps even revealed where each cell's core sat. Cells grew softer as their substrate stiffened, dropping from about 1050 pascals on plastic to 600 on a chitosan blend gel. Atomic force checks agreed, and a composite model pinned the softening on the cells' internal skeleton remodeling. The method suits delicate living samples that poking would disturb.
Catalog record
| CREATOR | Tatiana N Tikhonova, Anastasia V Barkovaya, Yuri M Efremov, Vugara V Mamed-Nabizade, Vasilii S Kolmogorov, Peter S Timashev, Nikolay N Sysoev, Victor V Fadeev, Petr V Gorelkin, Lihi Adler-Abramovich, Alexander S Erofeev, Evgeny A Shirshin, José Luis Toca-Herrera |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 50 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |