Screening and Validation of Stable Reference Genes for qRT-PCR Analysis in Epicauta gorhami (Coleoptera: Meloidae)
Guofeng Yang, Xuetao Yu, Yan Zhang, Jinhua Luo, Xiaofei Li, Li Zhu, Huanhuan Zhang, Lin Jin, Gang Wu, Xiaohong Yan, Chenhui Shen · 2024
Read Screening and Validation of Stable Reference Genes for qRT-PCR Analysis in Epicauta gorhami (Coleoptera: Meloidae) by Guofeng Yang, Xuetao Yu, Yan Zhang, Jinhua Luo, Xiaofei Li, Li Zhu, Huanhuan Zhang, Lin Jin, Gang Wu, Xiaohong Yan, Chenhui Shen (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11678893.txt.
Description
Guofeng Yang and co-authors pick stable reference genes for gene reads in a blister beetle in two thousand twenty-four. The beetle, Epicauta gorhami, feeds on soybeans as an adult and shifts shape oddly as it grows. Gene studies need anchor genes whose levels stay put, and none had been vetted for this species.
Ten housekeeping genes were checked with a gene-read method across adult ages, body parts, and temperatures, scored by five tools. Two genes, SOD and RPS18, held steadiest across ages and temperatures. A pair, RPS18 and RPS28, won for body parts. A test gene read with those anchors was richest in foregut, windpipe, and antenna, and poorest in gut sections and skin.
Catalog record
| CREATOR | Guofeng Yang, Xuetao Yu, Yan Zhang, Jinhua Luo, Xiaofei Li, Li Zhu, Huanhuan Zhang, Lin Jin, Gang Wu, Xiaohong Yan, Chenhui Shen |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 21 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |