Efficient Production of Recombinant Human Brain-Derived Neurotrophic Factor in Escherichia coli Through the Engineering of Its Pro-Region
Elisa Spaccapaniccia, Tiziano Cazzorla, Daniela Rossetti, Lucio De Simone, Maria Irene Antonangeli, Andrea Antonosante, Francesca Galli, Franca Cattani, Mariano Maffei, Franck Martin · 2024
Read Efficient Production of Recombinant Human Brain-Derived Neurotrophic Factor in Escherichia coli Through the Engineering of Its Pro-Region by Elisa Spaccapaniccia, Tiziano Cazzorla, Daniela Rossetti, Lucio De Simone, Maria Irene Antonangeli, Andrea Antonosante, Francesca Galli, Franca Cattani, Mariano Maffei, Franck Martin (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11678288.txt.
Description
Elisa Spaccapaniccia and co-authors describe, in a 2024 paper, a workable factory route for a human brain growth factor made in bacteria. The factor supports nerve health and draws wide research interest. Until now, no process could support making it at industrial scale.
A synthetic gene for the factor's precursor was placed in a common lab bacterium. The protein piled up in dense bundles inside the cells at high yield. The team's method refolded over half of that precursor into usable shape. An enzyme then trimmed it to the mature factor, and two cleaning steps purified it fully. A scalable recipe turns a scarce reagent into something industry can actually supply.
Catalog record
| CREATOR | Elisa Spaccapaniccia, Tiziano Cazzorla, Daniela Rossetti, Lucio De Simone, Maria Irene Antonangeli, Andrea Antonosante, Francesca Galli, Franca Cattani, Mariano Maffei, Franck Martin |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 38 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |