Production of Vespa tropica Hyaluronidase by Pichia pastoris
Piyapon Janpan, Bernhard Schmelzer, Anuwatchakij Klamrak, Patthana Tastub, Tewa Upathanpreecha, Shaikh Shahinur Rahman, Jaran Nabnueangsap, Yutthakan Saengkun, Prapenpuksiri Rungsa, Diethard Mattanovich, Sakda Daduang, Aaron Neiman · 2024
Read Production of Vespa tropica Hyaluronidase by Pichia pastoris by Piyapon Janpan, Bernhard Schmelzer, Anuwatchakij Klamrak, Patthana Tastub, Tewa Upathanpreecha, Shaikh Shahinur Rahman, Jaran Nabnueangsap, Yutthakan Saengkun, Prapenpuksiri Rungsa, Diethard Mattanovich, Sakda Daduang, Aaron Neiman (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11676926.txt.
Description
Piyapon Janpan and co-authors produce a hornet venom enzyme in yeast to avoid the folding problems of bacterial systems. The enzyme breaks down hyaluronic acid in tissues. Medicine and cosmetics both want reliable supplies of it.
Bacterial production left the protein tangled in clumps. The team rebuilt the gene for a yeast host instead. A methanol-driven switch produced about twice the earlier yield. Bioreactor runs secreted the enzyme into the broth. The product came out heavier than the plain sequence predicts. Sugar decorations added by yeast explain the extra mass. Working enzyme without harsh refolding is the prize. The route may scale for commercial supply.
Catalog record
| CREATOR | Piyapon Janpan, Bernhard Schmelzer, Anuwatchakij Klamrak, Patthana Tastub, Tewa Upathanpreecha, Shaikh Shahinur Rahman, Jaran Nabnueangsap, Yutthakan Saengkun, Prapenpuksiri Rungsa, Diethard Mattanovich, Sakda Daduang, Aaron Neiman |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 48 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |