Detection of miR-133a-5p Using a Molecular Beacon Probe for Investigating Postmortem Intervals
Eun Hye Lee, Mingyoung Jeong, Kwangmin Park, Dong Geon Lee, Eun Ju Lee, Haneul Lee, Ah Yeoung Kim, Jae Won Ahn, Hyun Jun Woo, Sunghyun Kim, Jaewon Lim, Jungho Kim · 2024
Read Detection of miR-133a-5p Using a Molecular Beacon Probe for Investigating Postmortem Intervals by Eun Hye Lee, Mingyoung Jeong, Kwangmin Park, Dong Geon Lee, Eun Ju Lee, Haneul Lee, Ah Yeoung Kim, Jae Won Ahn, Hyun Jun Woo, Sunghyun Kim, Jaewon Lim, Jungho Kim (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11676884.txt.
Description
Eun Hye Lee and co-authors build a glowing probe that estimates time since death from a tiny RNA in muscle, in this 2024 paper. Forensic teams need the postmortem interval early in an investigation. Small RNAs survive well after death, making them candidate clocks. The team designed a hairpin probe that lights up when it meets one muscle microRNA, miR-133a-5p, and tested it in mouse tissue kept cold or at room temperature.
The probe's glow rose in lockstep with the target's amount, down to a low detection floor. In the mouse muscle, the RNA's level stayed steady across ten days at both temperatures. That flat line, the authors note, suits it as a reference marker. The test finishes in thirty minutes after RNA extraction, suiting field use.
Catalog record
| CREATOR | Eun Hye Lee, Mingyoung Jeong, Kwangmin Park, Dong Geon Lee, Eun Ju Lee, Haneul Lee, Ah Yeoung Kim, Jae Won Ahn, Hyun Jun Woo, Sunghyun Kim, Jaewon Lim, Jungho Kim |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 20 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |