Mining and Characterization of Amylosucrase from Calidithermus terrae for Synthesis of α-Arbutin Using Sucrose
Anqi Li, Yamei He, Wenxuan Chen, Huimei Tao, Huawei Wu, Shaobin Li · 2024
Read Mining and Characterization of Amylosucrase from Calidithermus terrae for Synthesis of α-Arbutin Using Sucrose by Anqi Li, Yamei He, Wenxuan Chen, Huimei Tao, Huawei Wu, Shaobin Li (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11676761.txt.
Description
Anqi Li and five co-authors find and test an enzyme called amylosucrase from a hot spring germ, Calidithermus terrae. They use it to make alpha arbutin. The compound blocks the making of melanin in skin cells. It is prized in skin care, food, and drugs. But yields from plants are low. The safe, fast route is to build it with an enzyme, with cheap sucrose as the donor.
The team used probe chains to hunt like chains in germ data banks. Each match was a candidate enzyme. The genes were then made to work in Escherichia coli cells. One enzyme, named CtAs, made alpha arbutin well. The team tuned how it is made and how it works. They also tuned the build steps to lift the molar yield. The work adds a new enzyme source for large scale making.
Catalog record
| CREATOR | Anqi Li, Yamei He, Wenxuan Chen, Huimei Tao, Huawei Wu, Shaobin Li |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 49 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |