Chemically Induced Dimerization via Nanobody Binding Facilitates in Situ Ligand Assembly and On-Demand GPCR Activation
Shubhra · 2024
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Description
Saha and Cheloha (2024) built a method that assembles a working drug-like molecule on demand, right where it is needed. Building active molecules in place could give fine control over when and where a target is switched on, but most schemes need heavy engineering. Their approach uses nanobodies, small antibody fragments that grip short peptide tags with high precision, to hold molecular pieces next to each other.
They call the platform peptide epitope dimerization. Adding a two-tagged peptide linker pulls the nanobody conjugates together. The closeness triggers the pieces to join into a working ligand for a hormone receptor of the large GPCR family. Assembly also worked efficiently on the cell surface, with the template delivered by nanobodies. The authors say the scheme could generate active receptor ligands on demand at a chosen site in the body.
Catalog record
| CREATOR | Shubhra |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 39 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |