AND Logic Based on Suppressor tRNAs Enables Stringent Control of Sliding Base Editors in Pseudomonas putida
Elena Velázquez, Víctor de Lorenzo · 2024
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Description
Elena Velazquez and Victor de Lorenzo build a strict two-key genetic switch for a DNA base editor in a soil bacterium, in work published in 2024. Base editors rewrite single DNA letters and can diversify chosen genome regions, but loose control risks off-target changes. Standard switches failed to govern their editor, a deaminase fused to a T7 polymerase.
The team first broke the editor by inserting up to three stop codons into its gene. A suppressor tRNA, placed under a second chemical control, reads through those stops and restores the protein. The editor now fires only when both chemical inputs are present: a true AND gate. Tested in Pseudomonas putida, the two-layer control worked cleanly. The design suits controlled evolution of genes and pathways.
Catalog record
| CREATOR | Elena Velázquez, Víctor de Lorenzo |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 44 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |