A Biomolecular Circuit for Automatic Gene Regulation in Mammalian Cells with CRISPR Technology
Alessio Mallozzi, Virginia Fusco, Francesco Ragazzini, Diego di Bernardo · 2024
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Description
Alessio Mallozzi, Virginia Fusco, Francesco Ragazzini, and Diego di Bernardo built a gene circuit that holds protein levels steady in mammal cells in a 2024 paper. Cells in a lab dish face shifting conditions that make gene output drift. Their circuit pairs a CRISPR-based activator with an anti-CRISPR protein that restrains it.
The two parts bind each other in fixed proportion, which creates a feedback loop of the kind engineers call an antithetic integral controller. When output rises, the restraint grows; when it falls, the restraint eases. The result is near-perfect adaptation: the reporter protein stayed constant despite changed decay rates and DNA doses. The design can redirect to other genes.
Catalog record
| CREATOR | Alessio Mallozzi, Virginia Fusco, Francesco Ragazzini, Diego di Bernardo |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 38 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |