Protocol for open-source automated universal high-content multiplex fluorescence for RNA in situ analysis
Jean Descarpentrie, Florian Bernard, Wilfried Souleyreau, Lucie Brisson, Thomas Mathivet, Ioannis S Pateras, Océane CB Martin, Maria Lopez Chiloeches, Teresa Frisan · 2024
Read Protocol for open-source automated universal high-content multiplex fluorescence for RNA in situ analysis by Jean Descarpentrie, Florian Bernard, Wilfried Souleyreau, Lucie Brisson, Thomas Mathivet, Ioannis S Pateras, Océane CB Martin, Maria Lopez Chiloeches, Teresa Frisan (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11667704.txt.
Description
Seeing where each RNA message sits inside a cell takes staining and then heavy image work. Jean Descarpentrie and co-authors give an open-source protocol for that analysis in this 2024 paper. The staining method marks RNA in place with fluorescent tags. Up to fourteen different messages can be counted in a single image.
The steps start with finding each cell's nucleus using a macro in a free image tool. Counts then record how many cells carry each message, alone or in combination. The protocol also covers storing raw data and quality check images. A small Python app gathers all counts into one spreadsheet. Labs without commercial software can now run high-content RNA imaging end to end.
Catalog record
| CREATOR | Jean Descarpentrie, Florian Bernard, Wilfried Souleyreau, Lucie Brisson, Thomas Mathivet, Ioannis S Pateras, Océane CB Martin, Maria Lopez Chiloeches, Teresa Frisan |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 78 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |