Optimized reporters for multiplexed detection of transcription factor activity
Max Trauernicht, Teodora Filipovska, Chaitanya Rastogi, Bas van Steensel · 2024
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Description
Max Trauernicht and co-authors present a 2024 journal article on better reporters for transcription factor activity. Dozens of these DNA-binding proteins run the genome in any cell, yet tools to watch many at once are clumsy. The team used a massively parallel reporter assay to tune reporter designs for 86 factors, then checked how specific each design stayed across many perturbation tests.
Clear design rules emerged, and for 62 factors the tuned reporters beat what was available in both sensitivity and specificity. The authors offer the prime reporter set as a kit for mapping regulatory networks and tracing signaling pathways in living cells.
Catalog record
| CREATOR | Max Trauernicht, Teodora Filipovska, Chaitanya Rastogi, Bas van Steensel |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 83 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |