A Photo‐Caged Cross‐Linker for Identifying Protein‐Protein Interactions
Xingyu Chen, Cristina Jiménez López, André Nadler, Florian Stengel · 2024
Read A Photo‐Caged Cross‐Linker for Identifying Protein‐Protein Interactions by Xingyu Chen, Cristina Jiménez López, André Nadler, Florian Stengel (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11664911.txt.
Description
Xingyu Chen and co-authors report a 2024 chemistry tool for catching proteins in the act of touching. Mapping protein partnerships inside living cells is hard because common cross-linkers leak poorly through cell walls and waste themselves on water. The team built a cross-linker caged by light-sensitive groups that keep it stable and fat-friendly until a flash of ultraviolet frees it.
Once freed, the linker grabs nearby amino groups and locks the bond with a follow-up step. On a test protein, the tool reliably flagged known partnerships under mass spectrometry. It also slipped into intact HeLa cells, which points to future use for mapping partnerships in their true home.
Catalog record
| CREATOR | Xingyu Chen, Cristina Jiménez López, André Nadler, Florian Stengel |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 26 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |