An improved method for whole-mount in situ hybridization in regenerating tails of Xenopus laevis tadpoles
A. D. Shitikov, E. A. Parshina, A. G. Zaraisky, M. B. Tereshina · 2024
Read An improved method for whole-mount in situ hybridization in regenerating tails of Xenopus laevis tadpoles by A. D. Shitikov, E. A. Parshina, A. G. Zaraisky, M. B. Tereshina (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11663889.txt.
Description
Shitikov and three co-authors improved a lab method for seeing where genes switch on inside regrowing frog tails, in a 2024 paper. The method stains whole tissue so gene activity shows in place. Tadpoles of the frog Xenopus laevis regrow a lost tail within a week, which makes them a favorite model for regeneration studies.
New sequencing work has named the cell types and genes at play in the regrowing tail, including repair cells marked by one key gene. Checking those results with the staining method is hard, since faint signals, deep tissue, and background stains get in the way. The team added treatment steps that cut background and sharpen the view of stained cells. Their tuned protocol gives clearer maps of when and where target genes act during regrowth.
Catalog record
| CREATOR | A. D. Shitikov, E. A. Parshina, A. G. Zaraisky, M. B. Tereshina |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 50 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |