Developing robust quantitative PCR primers for comparative biomass analysis of Tall Fescue (Festuca arundinacea) and its Epichloë endophyte
Darrian Talamantes, Caitlin Kirkpatrick, Jason Wallace · 2024
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Description
Darrian Talamantes, Caitlin Kirkpatrick, and Jason Wallace develop measurement tools for a grass and its fungal partner in a 2024 paper. Tall fescue owes much of its toughness to a fungus living inside it, which shields the grass from stress. Existing tests only check whether the fungus is present, not how much of it there is.
The team compared several primer sets, the short DNA anchors used in quantitative PCR, for both the grass and the fungus. They report standard curves, fit quality, and efficiency for every candidate. The best sets allow side by side biomass estimates of host and partner. Such measurement matters for breeding and managing fescue pastures where the fungus's benefits and risks both scale with its amount.
Catalog record
| CREATOR | Darrian Talamantes, Caitlin Kirkpatrick, Jason Wallace |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 9 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |