Simultaneous determination of cytosolic aminoacyl-tRNA synthetase activities by LC–MS/MS
Marisa I Mendes, Nicole I Wolf, Joëlle Rudinger-Thirion, Dominic Lenz, Magali Frugier, Patrick Verloo, Hanna Mandel, Joshua Manor, Rachel Kassel, Willemijn E Corpeleijn, Sanne van der Rijt, Elsbeth M Schroor, Silvy J M van Dooren, Christian Staufner, Gajja S Salomons, Desirée E C Smith · 2024
Read Simultaneous determination of cytosolic aminoacyl-tRNA synthetase activities by LC–MS/MS by Marisa I Mendes, Nicole I Wolf, Joëlle Rudinger-Thirion, Dominic Lenz, Magali Frugier, Patrick Verloo, Hanna Mandel, Joshua Manor, Rachel Kassel, Willemijn E Corpeleijn, Sanne van der Rijt, Elsbeth M Schroor, Silvy J M van Dooren, Christian Staufner, Gajja S Salomons, Desirée E C Smith (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11662674.txt.
Description
Marisa Mendes and co-authors present a lab method from 2024 that can test many enzymes at once in a single cell sample. The enzymes, called aminoacyl-tRNA synthetases, help build proteins, and faults in their genes can cause disease. Signs of these faults can overlap, so gene tests alone often leave cases unclear.
The new test uses a form of mass spectrometry to measure the work of all such enzymes in the cell sap at one time. The team checked the method and then tried it on samples from five people with faults in distinct genes. Their results show the test works in practice and can help confirm a diagnosis and judge new gene changes.
Catalog record
| CREATOR | Marisa I Mendes, Nicole I Wolf, Joëlle Rudinger-Thirion, Dominic Lenz, Magali Frugier, Patrick Verloo, Hanna Mandel, Joshua Manor, Rachel Kassel, Willemijn E Corpeleijn, Sanne van der Rijt, Elsbeth M Schroor, Silvy J M van Dooren, Christian Staufner, Gajja S Salomons, Desirée E C Smith |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 41 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |