Efficient suppression of premature termination codons with alanine by engineered chimeric pyrrolysine tRNAs
Aya Awawdeh, Alejandro Tapia, Sarah A Alshawi, Olabode Dawodu, Sarah A Gaier, Caitlin Specht, Jean-Denis Beaudoin, Jeffery M Tharp, Oscar Vargas-Rodriguez · 2024
Read Efficient suppression of premature termination codons with alanine by engineered chimeric pyrrolysine tRNAs by Aya Awawdeh, Alejandro Tapia, Sarah A Alshawi, Olabode Dawodu, Sarah A Gaier, Caitlin Specht, Jean-Denis Beaudoin, Jeffery M Tharp, Oscar Vargas-Rodriguez (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11662663.txt.
Description
A premature stop signal inside a gene can truncate a vital protein and cause severe disease. Awawdeh and co-authors engineer transfer RNAs that read through such stops in a 2024 paper, starting from a natural tRNA that already ignores one stop type.
That scaffold, borrowed from an archaeal system, inserts its amino acid where the faulty stop sits. The team built a family of chimeric variants tuned to deliver alanine specifically. Careful engineering aimed to raise both the strength and the accuracy of the read-through, the pair of traits earlier attempts struggled to combine. Success would open one drug platform for many different genetic diseases that share the same error type. The work reports the design and its early performance.
Catalog record
| CREATOR | Aya Awawdeh, Alejandro Tapia, Sarah A Alshawi, Olabode Dawodu, Sarah A Gaier, Caitlin Specht, Jean-Denis Beaudoin, Jeffery M Tharp, Oscar Vargas-Rodriguez |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 70 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |