Optogenetic control of Corynebacterium glutamicum gene expression
Chen Deng, Ruijie Xin, Xingjian Li, Jie Zhang, Liqiang Fan, Yongjun Qiu, Liming Zhao · 2024
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Description
Deng and co-authors switch genes on and off with light inside an industrial bacterium, in a 2024 biotechnology study. Corynebacterium glutamicum is a factory workhorse for amino acids and chemicals, but balancing growth against production needs nimble control. Chemical switches can be toxic or blunt. The team built the first optogenetic systems for this species, using light-sensitive RNA-binding proteins to form light-driven transcription factors, plus a light-guided gene silencing system.
With light as the dial, they steered the cell's metabolism to make two valuable sugar chains for the first time in this host. A light-controlled fermenter reached the highest reported yield of one product, 6.2 grams per liter. The circuits are programmable and portable to other production strains, the authors argue.
Catalog record
| CREATOR | Chen Deng, Ruijie Xin, Xingjian Li, Jie Zhang, Liqiang Fan, Yongjun Qiu, Liming Zhao |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 64 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |