A systematic quantitative approach comprehensively defines domain-specific functional pathways linked to Schizosaccharomyces pombe heterochromatin regulation
Abubakar Muhammad, Zsuzsa Sarkadi, Agnisrota Mazumder, Anissia Ait Saada, Thomas van Emden, Matias Capella, Gergely Fekete, Vishnu N Suma Sreechakram, Bassem Al-Sady, Sarah A E Lambert, Balázs Papp, Ramón Ramos Barrales, Sigurd Braun · 2024
Read A systematic quantitative approach comprehensively defines domain-specific functional pathways linked to Schizosaccharomyces pombe heterochromatin regulation by Abubakar Muhammad, Zsuzsa Sarkadi, Agnisrota Mazumder, Anissia Ait Saada, Thomas van Emden, Matias Capella, Gergely Fekete, Vishnu N Suma Sreechakram, Bassem Al-Sady, Sarah A E Lambert, Balázs Papp, Ramón Ramos Barrales, Sigurd Braun (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11662645.txt.
Description
Abubakar Muhammad leads a team of thirteen authors mapping which genes guard the silent regions of the yeast genome. They map them domain by domain. Heterochromatin silences genes and protects genome integrity. But its control differs across domains. In fission yeast, those domains are the pericentromeres, the subtelomeres, and the silent mating-type locus. The team paired quantitative silencing reporters for each domain with iterative genetic screening.
Three hundred sixty-nine mutants showed reduced or enhanced silencing. Mutations in the core machinery weakened silencing in all domains, as expected. Most other mutants hit specific domains only. Metabolic pathways, for instance, mainly affected subtelomeres. Profiles that looked alike grouped subunits of the same complexes. One protein not yet characterized also stood out from the screen. The result is a functional atlas of silencing control. It shows which parts are shared and which are local to a domain, in fine grain.
Catalog record
| CREATOR | Abubakar Muhammad, Zsuzsa Sarkadi, Agnisrota Mazumder, Anissia Ait Saada, Thomas van Emden, Matias Capella, Gergely Fekete, Vishnu N Suma Sreechakram, Bassem Al-Sady, Sarah A E Lambert, Balázs Papp, Ramón Ramos Barrales, Sigurd Braun |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 102 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |