Loop-mediated isothermal amplification assays for the detection of antimicrobial resistance elements in Vibrio cholera
Daniel Antonio Negrón, Shipra Trivedi, Nicholas Tolli, David Ashford, Gabrielle Melton, Stephanie Guertin, Katharine Jennings, Bryan D Necciai, Shanmuga Sozhamannan, Bradley W Abramson · 2024
Read Loop-mediated isothermal amplification assays for the detection of antimicrobial resistance elements in Vibrio cholera by Daniel Antonio Negrón, Shipra Trivedi, Nicholas Tolli, David Ashford, Gabrielle Melton, Stephanie Guertin, Katharine Jennings, Bryan D Necciai, Shanmuga Sozhamannan, Bradley W Abramson (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11657800.txt.
Description
Daniel Negrón led a team building fast tests for drug resistance in cholera bacteria in a 2024 paper. These bacteria can pick up genes that defeat many drugs, and knowing that quickly helps doctors avoid useless antibiotics. A color-based test method works without special gear and finishes in under half an hour, suiting field clinics.
Designing such tests is hard, so the team built software that drafts test primers and checks them against large gene databases. A vision app then reads the color change in a measured way, removing guesswork. Of the tests they tried in the lab, just over half gave a clear positive signal by both eye and software.
Catalog record
| CREATOR | Daniel Antonio Negrón, Shipra Trivedi, Nicholas Tolli, David Ashford, Gabrielle Melton, Stephanie Guertin, Katharine Jennings, Bryan D Necciai, Shanmuga Sozhamannan, Bradley W Abramson |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 34 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |