Saliva microbiome profiling by full-gene 16S rRNA Oxford Nanopore Technology versus Illumina MiSeq sequencing
Anders Esberg, Niklas Fries, Simon Haworth, Ingegerd Johansson · 2024
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Description
Reading the mouth's microbes species by species needs a sequencer that can spell a whole gene, not just a fragment. Anders Esberg and co-authors benchmarked nanopore full-gene sequencing of saliva against the standard short-read method in 2024. They varied how samples were collected and extracted, how little DNA was used, and which software and databases did the naming.
The long-read method named oral species and subspecies more sharply than the short-read one, though the software choices swayed results a lot. Spit stored in population biobanks proved fit for large studies. The authors say the approach can map mouth bacteria tied to diseases, including rare ones, with new confidence.
Catalog record
| CREATOR | Anders Esberg, Niklas Fries, Simon Haworth, Ingegerd Johansson |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 53 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |