Rapid detection of SARS-CoV-2 RNA using a one-step fast multiplex RT-PCR coupled to lateral flow immunoassay
Insaf Bel Hadj Ali, Hejer Souguir, Mouna Melliti, Mohamed Vall Taleb Mohamed, Monia Ardhaoui, Kaouther Ayouni, Sondes Haddad-Boubaker, Yusr Saadi Ben Aoun, Henda Triki, Ikram Guizani · 2024
Read Rapid detection of SARS-CoV-2 RNA using a one-step fast multiplex RT-PCR coupled to lateral flow immunoassay by Insaf Bel Hadj Ali, Hejer Souguir, Mouna Melliti, Mohamed Vall Taleb Mohamed, Monia Ardhaoui, Kaouther Ayouni, Sondes Haddad-Boubaker, Yusr Saadi Ben Aoun, Henda Triki, Ikram Guizani (2024) in the Tower of Babel, a free online library of public-domain works preserved in clean, searchable text. No sign-up. The full text is served as a plain-text file at a predictable URL, where text is present: https://antarctic-labs.com/tower-of-babel/pmc-11653822.txt.
Description
Insaf Bel Hadj Ali and co-authors build a quick visual test for the virus that causes COVID-19, aimed at labs with limited supplies. Their method joins a fast one-step PCR step, which copies two parts of the viral genome at once, with a strip device that shows the result as visible lines. The work was published in 2024.
The team screened primer pairs against five viral gene targets and kept two clean pairs, for the N and E genes. On 50 known positive and 50 negative nose swab samples, the combined test caught 88 percent of positives and cleared 98 percent of negatives. Every sample with a strong viral signal tested positive, while weak samples mostly read negative. The authors present the assay as proof that a cheap, fast, equipment-light test can work for diagnosis and surveillance.
Catalog record
| CREATOR | Insaf Bel Hadj Ali, Hejer Souguir, Mouna Melliti, Mohamed Vall Taleb Mohamed, Monia Ardhaoui, Kaouther Ayouni, Sondes Haddad-Boubaker, Yusr Saadi Ben Aoun, Henda Triki, Ikram Guizani |
|---|---|
| YEAR | 2024 |
| COLLECTION | PAPERS |
| FORMAT | TXT |
| FILE SIZE | 47 KB |
| VERSION | 1.0 |
| LICENSE | CC BY 4.0 |
| RIGHTS | CC BY |
| SOURCE | Europe PMC |